Quantification of Methotrexate in Human Serum Using Surface-Enhanced Raman Scattering—Toward Therapeutic Drug Monitoring

Therapeutic drug monitoring (TDM) can improve clinical care when using drugs with pharmacokinetic variability and a narrow therapeutic window. Rapid, reliable, easy-to-use detection methods are required in order to decrease the time of analysis also enable TDM resource-limited settings or even at bedside. Monitoring methotrexate (MTX), an anticancer drug, is critical since it needed follow clearance rate decide how administer rescue leucovorin (LV), avoid toxicity death. We show that optimized nanopillar-assisted separation (NPAS) method surface-enhanced Raman scattering, we were able measure MTX PBS serum linear range 5–150 μM confirmed be carried out presence LV. Additionally, NPAS was combined centrifugal filtration, quantification limit 2.1 for human sample achieved. The developed enables fast (10 min) from (>90% accuracy). Furthermore, potential TDM, quantifying samples, collected patients who undergoing high-dose therapy.